Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...

Scientists from the United States have recently identified a novel approach to rapidly and accurately detect the omicron BA.2.12.1 sub-lineage of severe acute respiratory syndrome coronavirus 2 ...

A new form of PCR has been used to improve the library preparation for forensic DNA typing from difficult samples like touch evidence and human remains. Typically, DNA sequencing and analysis is ...

Failed polymerase chain reactions (PCRs) are a common cause of frustration in the lab. While a typical PCR protocol is quite straightforward, scientists must optimize each step to amplify the intended ...

Limited sample material and insufficient DNA input pose significant challenges for downstream analysis in various laboratory settings. To overcome this issue, isothermal amplification techniques have ...

In the January 25 Nature, Iyer et al. describe an elegant technique to identify transcriptional target genes throughout the genome (Nature 2001, 409:533-538). They combined chromatin ...