Before nuclear pore complexes were well understood, it was unclear whether nuclear proteins diffused passively into the nucleus and accumulated there by binding to residents of the nucleus such as chromosomes, or were actively transported and accumulated regardless of their affinity for nuclear components. A classic experiment that addressed this problem used several forms of radioactive nucleoplasmin, which is a large Figure Q 12-1 Cell location of injected nucleoplasmin and nucleoplasmin compo
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